Polymerase chain reaction-restriction fragment length polymorphism of ribosomal internal transcribed spacer region analysis on polyacrylamide gel electrophoresis reveals two haplotypes coexisting inMyzus persicae

2002 ◽  
Vol 23 (2) ◽  
pp. 186-188 ◽  
Author(s):  
Raboudi Faten ◽  
Marrakchi Mohamed ◽  
Makni Mohamed
Keyword(s):  
Polymerase Chain Reaction ◽  
Restriction Fragment Length Polymorphism ◽  
Gel Electrophoresis ◽  
Internal Transcribed Spacer ◽  
Fragment Length Polymorphism ◽  
Polyacrylamide Gel Electrophoresis ◽  
Chain Reaction ◽  
Internal Transcribed Spacer Region ◽  
Polymerase Chain ◽  
Restriction Fragment Length

scholarly journals Internal Transcribed Spacer Sequence-Based Phylogeny and Polymerase Chain Reaction-Restriction Fragment Length Polymorphism Differentiation of Tilletia walkeri and T. indica

2001 ◽  
Vol 91 (10) ◽  
pp. 935-940 ◽  
Author(s):  
Laurene Levy ◽  
Lisa A. Castlebury ◽  
Lori M. Carris ◽  
Robert J. Meyer ◽  
Guillermo Pimentel
Keyword(s):  
Polymerase Chain Reaction ◽  
Restriction Fragment Length Polymorphism ◽  
Restriction Fragment ◽  
Length Polymorphism ◽  
Fragment Length ◽  
Internal Transcribed Spacer ◽  
Fragment Length Polymorphism ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Restriction Fragment Length

A polymerase chain reaction-restriction fragment length polymorphism assay to distinguish Tilleita walkeri, a rye grass bunt fungus that occurs in the southeastern United States and Oregon, from T. indica, the Karnal bunt fungus, is described. The internal transcribed spacer (ITS) region of the ribosomal DNA repeat unit was amplified and sequenced for isolates of T. indica, T. walkeri, T. horrida, and a number of other taxa in the genus Tilletia. A unique restriction digest site in the ITS1 region of T. walkeri was identified that distinguishes it from the other taxa in the genus. Phylogenetic analysis of the taxa based on ITS sequence data revealed a close relationship between T. indica and T. walkeri, but more distant relationships between these two species and other morphologically similar taxa.

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